Glycoprotein analysis can involve identifying complex N and 0-Linked structures composed of frequently similar and repeating sugar moieties. Hydrophilic-interaction liquid chromatography (HILIC) is a well-recognized and reliable technique that effectively separates and quantitates isolated glycans after their derivatization with reagents fluorescent labels. Waters ACQUITY UPLC® BEH Glycan column was specifically developed and QC tested to provide superior UPLC® component resolution in less time for the wide range of glycan structures. When coupled to Waters ACQUITY UPLC system, our solid-phase sample preparation and new column chemistry are ideally suited to help users get the correct answers faster.
ACQUITY UPLC BEH Glycan Column Separation of 2-AB Labeled Human IgG Glycans
The N-linked glycans from pooled human IgG contain high mannose as well as neutral, and sialylated complex structures. The chromatogram shows 35 minutes of a one hour analysis. This challenging sample includes high mannose, bisecting GlcNAc and sialylated species.