Glycan Separation Technology Columns : Waters

Glycoprotein analysis can involve identifying complex N and 0-Linked structures composed of frequently similar and repeating sugar moieties. Hydrophilic-interaction liquid chromatography (HILIC) is a well-recognized and reliable technique that effectively separates and quantitates isolated glycans after their derivatization with reagents fluorescent labels. Waters ACQUITY UPLC^® BEH Glycan column was specifically developed and QC tested to provide superior UPLC^® component resolution in less time for the wide range of glycan structures. When coupled to Waters ACQUITY UPLC system, our solid-phase sample preparation and new column chemistry are ideally suited to help users get the correct answers faster.

Based on Waters BEH particle and bonding technology for stable and reproducible labeled glycan separations
Improved component resolution in less time compared to existing HPLC-based methods
Optimized for use with ACQUITY UPLC system with fluorescence detection
Quality-control tested with relevant labeled glycan standards for consistent batch-to-batch column performance

ACQUITY UPLC BEH Glycan Column Separation of 2-AB Labeled Human IgG Glycans

The N-linked glycans from pooled human IgG contain high mannose as well as neutral, and sialylated complex structures. The chromatogram shows 35 minutes of a one hour analysis. This challenging sample includes high mannose, bisecting GlcNAc and sialylated species.

Glycan Separation Technology Columns

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