The 1.7 µm Charged Surface Hybrid (CSH™) particle is Waters third generation hybrid particle technology. Based on Waters Ethylene Bridged Hybrid (BEH) particle technology, CSH particles incorporate a low level surface charge that improves sample loadability and peak asymmetry in low-ionic-strength mobile phases, while maintaining the mechanical and chemical stability inherent in the BEH particle technology.
The advantages of CSH Technology UPLC chromatography columns include:
Click here for Waters new CSH130 columns for peptide separations.
CSH particle technology is also available in HPLC particle sizes (3.5 and 5 µm) in the XSelect™ family of HPLC columns, enabling seamless transfer between HPLC and UPLC® technology platforms.
The CSH C18 column provides exceptional peak shape, particularly for basic compounds under low-pH, weak-ionic-strength mobile-phase conditions.
Improved Loading Capacity
CSH Technology provides exceptional loading capacity, particularly for basic compounds under low-pH, weak-ionic-strength mobile phase conditions. The increased and improved loadability possible with CSH Technology permits the separation, identification and quantitation of closely eluting impurities or degradants.
The CSH Technology fluoro-phenyl and phenyl phases offer the ability to retain polar analytes. Selectivity is manipulated through the use of pH, mobile-phase buffer strength, organic modifier and chromatography column choice.
UPLC to HPLC Method Transfer
The CSH Technology particles used in both ACQUITY UPLC and XBridge HPLC columns differ only in their particle size, enabling seamless methods transfer and the fastest route to an optimized LC method. CSH particles maximize efficiency, improve high-pH stability and improve chromatography column reliability for both UPLC and HPLC systems.