For over 50 years, Waters has been the market leader in GPC and SEC analysis, providing the highest quality columns and applications support for both organic- and aqueous-based size separations. Refinements in packing materials and technology have allowed industry scientists to expand the original GPC/SEC polymer separations to include applications for separating small and large molecules from interfering matrices, such as those found in foods, pharmaceutical preparations, and natural products.
Waters leading GPC/SEC brands include:
Gel-permeation chromatography, size-exclusion chromatography, and gel-filtration chromatography are synonymous techniques used to resolve component mixtures based on their size in solution. When evaluating the column choices in the table (below), please consider these column properties that will influence the separation:
Waters HSPgel™ AQ SEC Columns are optimized for high-speed polymer analysis in aqueous solution. These columns reduce solvent consumption, increase throughput, and provide accurate molecular weight data for any room temperature analysis.
Designed particularly for low molecular weight samples, the Waters Styragel® HR Columns are ideal for the analysis of oligomers, epoxies, and polymer additives where high resolution is critical. Packed with rigid 5 µm particles, these columns deliver unrivaled resolution and efficiency in the low-to-mid molecular weight range.
The HSPgel™ HR Column series is designed for high resolution, room termperature, organic polymer GPC. These columns are packed in THF and can be converted once to toluene, methylene chloride, or chloroform.
The Styragel® HT Columns can be used with aggressive solvents at high temperatures without sacrificing resolution or column lifetime. Packed with rigid 10 µm particles, they have a typical plate count greater than 10,000 plates per column. These columns are extremely durable due to a narrow particle size distribution that results in a very stable column bed. Suitable for both ambient- and high-temperature analysis. The Styragel HT Columns offer excellent resolution of polymers in the mid-to-high molecular weight range.
The HSPgel™ HT Columns are designed for room-temperature to high-temperature (180 °C) organic GPC. The columns come shipped in either THF or ODCB. The ODCB packed column should be used for direct conversion to TCB. These columns can withstand multiple solvent switches.
The Styragel® HMW Columns were specifically designed for the analysis of ultra-high molecular weight polymers susceptible to shearing. Combining high-porosity 10 µm frits and 20 µm particles, the Styragel HMW Columns minimize polymer shear effects. These state-of the-art columns can be used at ambient or elevated temperatures, and exhibit excellent column lifetime.
The HSPgel™ RT Columns are designed for room temperature, routine work of organic polymer GPC. These come packed in THF and can be converted multiple times from THF to toluene, chloroform, methylene chloride, DMF, DMSO, etc.
The Envirogel™ High-Efficiency GPC Cleanup Columns are specifically designed to remove low volatility, high molecular weight interferences, such as lipids and natural resins, from environmental samples as specified in EPA Method 3640A. In the past, the cleanup procedure for environmental samples was performed on low-efficiency GPC columns based on packing particle diameters of 37-75 µm (200 to 400 mesh) Bio-Beads® S-Z resins. The high-efficiency Envirogel GPC Cleanup Columns increase the speed of this process while simultaneously reducing solvent consumption.
Packed with hydroxylated polymethacrylate-based gel, Waters Ultrahydrogel™ SEC Columns are ideal for the analysis of aqueous-soluble samples, such as oligomers; oligosaccharides; polysaccharides; and cationic, anionic, and amphoteric polymers. Measuring 7.8 x 300 mm, these high-resolution columns offer many advantages over conventional aqueous SEC columns:
Shodex® Columns are high-efficiency columms for GPC analysis. Featuring a very reproducible styrene and divinylbenzyne technology, these columns have more than twenty years of history and have been used by customers all over the world.
Ultrastyragel™ Preparative Columns provide high-efficiency GPC separations for compound isolation and sample cleanup. Closely related to Styragel® GPC Columns, the family of Ultrastyragel Columns provide a two- to three-fold increase in efficiency (plates/meter) that improves separation speed and reduces solvent consumption for preparative isolation. Separations that once required several smaller Styragel Columns can be performed on a single, more efficient Ultrastyragel Preparative Column.