Protein Reversed-Phase Columns

Reversed-Phase Chromatography

Reversed-phase chromatography of proteins, done with columns consisting of wide-pore size particles ( e.g, 300Å) functionalized with short-ligand length chemistries, is a separation technique based on the relative hydrophobic characteristics of the proteins in solution. Gradients of increasing organic solvent concentration are frequently used to affect separations in the presence of ion-pairing reagents(e.g., 0.1% TFA) that minimize undesired ionic interactions. In general, the hydrophobicity of the protein or protein sub-unit determines the elution order, with the least hydrophobic proteins eluting first. Factors such as particle composition (silica vs hybrids), pore size, ligand type and density, as well as separation conditions (e.g., gradient duration, separation temperature, flow rate) all play an important role in obtaining a separation that meets application requirements.

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Bioseparations and Analyses Catalog 2017/18 Bioseparations Catalog

HPLC, UHPLC, UPLC, and LC-MS consumables for biomolecule analyses.
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