The evaluation of pharmaceutical raw materials and finished products for impurities and degradation products is an essential part of the drug development and manufacturing testing process. Additionally, toxicological information must be obtained on any drug-related impurity that is present at a concentration of greater than 0.1% of that of the active pharmaceutical ingredient (API).
In pharmaceutical QC and manufacturing, impurity analysis has traditionally been performed by HPLC with UV, PDA, or MS detection. As it is essential to detect and measure all of the impurities in the sample, it is necessary to have a high resolution separation process. This usually involves long analysis times resulting in low throughput.
As candidate pharmaceutical compounds become more potent and are dosed at lower and lower levels, ever more sensitive assays are needed to detect and measure impurities. The low throughput of HPLC can become the rate-limiting step in product release testing or process evaluation. Since much of the process of impurity identification involves the coupling of LC to sophisticated MS, any reduction in analysis time will result in a more efficient use of these significant investments.
Analytical technology advances such as UPLC and UPC2 offer significant improvements in throughput and sensitivity, with benefits to the process of product release and identification of drug-related impurities.
Profiling, detecting, and quantifying drug substances and their impurities in raw materials and final product testing is an essential part of the drug development and formulation process.
The ACQUITY UPLC System streamlines the batch analysis of raw materials quality assessment. Here, the analysis of four different batches of budesonide from different suppliers reveals that there are significant differences in the number and magnitude of the impurity peaks in each batch sample.
UPLC and Impurities in Batch Analysis