The GlycoWorks Sample Preparation consumable line along with the ACQUITY UPLC BEH Glycan Separation Technology Column is optimized for use with ACQUITY UPLC systems with fluorescence detection (FLR) for separating the released glycans of biopharmaceuticals as their 2-aminobenzamide (2-AB) derivatives.
The GlycoWorks Sample Preparation Consumable Line includes the following features:
The HILIC Advantage
Hydrophilic Interaction Chromatography (HILIC) can be used to successfully improve retention of very polar species. This technique is rapidly growing for this application area. One reason for this is that this mode of separation is well suited for glycans due to their highly polar nature. The GlycoWorks HILIC SPE provides an efficient and reproducible mechanism for effective glycan cleanup and provides quantitative and consistent recoveries for a diverse range of N-Glycans.
The GlycoWorks Kit offers a protocol for complete sample processing of released N-glycans with either 2-AB or 2-AA labeling. Two formats offered, one for high-throughput which is a 96-well plate and the other for low volume users in a 1 cc cartridge format.
Kit components include:
There is no “one size fits all” solution for released glycan analysis. Due to the complicated nature of the sample preparation process compounded by the complexity of customer samples, product support is critical. The GlycoWorks protocol consists of the following to help aid in customer questions and ease of use when following the protocol:
Only one company can provide you with everything you need for released glycan analysis. The GlycoWorks Sample Preparation consumable line was developed and designed to complement the existing Waters Glycan Application Solution on 2-AB labeled N-Glycan, this sample preparation offering and complimentary standards provides a unique, comprehensive, yet versatile, solution.