Why is sample volume important?
Autosamplers include different injector needle designs, such as bottom or side draw, and are programmed to have the needle stop before the bottom of the vial, preventing needle damage and vial breakage. The needle design and final placement above the vial bottom leaves a residual volume of sample that is not accessible for injection. Some vials are designed for limited sample volume applications that give access to more of the sample. The amount of sample available and the need to access limited residual volume will therefore have a significant impact on the most appropriate vial to be used.