What type of needle are you using?

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How to determine the needle type for your instrument?

For all the ACQUITY UPLC, nano ACQUITY UPLC and ACQUITY I-Class FL (fixed loop needles), you must identify the needle type being used to choose the most appropriate plate and seal selection. All other systems presented in this product selector uses metal needles as a default.

If you are not sure of the needle that is used in your system, follow the procedure below to identify it.

The needle is plumbed into the injector valve in the Sample Manager. The injector valve is located on the right, front side of the sample manager. Open the door (also called a drawer) on the right, front side of the Sample Manager to view the injector valve. The needle will be plumbed into one of the ports in the valve. System Port for Sample Needle Determine the port for the needle by referring to the table for the ACQUITY UPLC System being used. Look at the color of the tubing near the fitting in the identified port. The color of the tube indicates what type of needle is used. There may be a heat shrink band over the tube near the fitting in the port. You should still be able to see some of the tubing before it enters sample compartment of the Sample Manager. From the table below, the tubing color indicates the needle type for Sample Needle

SystemPort for Needle
nanoACQUITY UPLC 2
ACQUITY UPLC FL 3
ACQUITY UPLC I-Class FL 3
ACQUITY UPC2 FL 3
FL: Fixed Loop Needle, FTN: Flow Through Needle

Are your samples volume limited?

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Why is sample volume important?

The wells need to be large enough to hold the volume of the sample. In situations where the sample volume is limited and most of the volume is needed for injection, the profile of the well bottom is critical. Wells with steep angles or conical bottoms ensure that the sample will drain to the center. Pooling the limited sample in a deeper center allows the equipment greater access, leaving less residual sample behind.

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    Detailed Specs
    Number of Wells
    Well Volume
    Well Shape
    Material
    Approximate Residual Volume
    Number of Plates for Sample Manager
    Total number of Samples in Sample Manager
    Number of Plates for Sample Organizer
    Total number of Samples in Sample Organizer