Reversed-Phase Method Validation Kit

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186005463 - XBridge Peptide BEH C18 Method Validation Kit, 130Å, 5 µm, 4.6 mm X 100 mm, 3/pkg

Part Number: 186005463

The reproducibility of a chromatographic column has a critical impact on the long-term reliability and robustness of an analytical method, and its reproducibility lies completely outside the user's control. With exceptional batch-to-batch and column-to-column reproducibility, Waters well-established particle and column manufacturing process control provides confidence in the long-term reliability of your analytical method. XBridge BEH130 C18 5um Method Validation Kit includes three batches of chromatographic media [derived from different base particles] to judge the quality, reliability, and consistency of your chromatographic method. Each batch of selected XBridge BEH130 C18 5um chemistry is specifically QC tested with a mixture of carefully selected peptide standards to help ensure consistent performance.

186005463 - XBridge Peptide BEH C18 Method Validation Kit, 130Å, 5 µm, 4.6 mm X 100 mm, 3/pkg
Part Number: 186005463
Brand: XBridge

Products > Chromatography Consumables & Columns > Columns > Bioseparations Columns & Consumables > Peptide Separations > Peptide Columns
% Carbon Load 18
Application Peptide
Chemistry C18
Endcapped No
Endfitting Type Waters
Format Method Validation Kit
ID 4.6 mm
Length (mm) 100 mm
Mode Reversed-Phase
Particle Shape Spherical
Particle Size (dp) 5 µm
Particle Substrate Hybrid
Pore Size 130Å
QC Tested Peptide
Silanol Activity Low
Surface Area 185
System HPLC
Technology BEH
USP Classification L1
Units in Package 3/pkg
pH Range 1 - 12

info

186005465 - XBridge Protein BEH C4 Method Validation Kit, 300Å, 3.5 µm, 4.6 mm X 100 mm, 3/pkg

Part Number: 186005465

The reproducibility of a chromatographic column has a critical impact on the long-term reliability and robustness of an analytical method, and its reproducibility lies completely outside the user's control. With exceptional batch-to-batch and column-to-column reproducibility, Waters well-established particle and column manufacturing process control provides confidence in the long-term reliability of your analytical method. XBridge BEH300 C4, 3.5um Method Validation Kit includes three batches of chromatographic media [derived from different base particles] to judge the quality, reliability, and consistency of your chromatographic method. Each batch of selected XBridge BEH300 C18, 3.5um chemistry is specifically QC tested with a mixture of carefully selected protein standards to help ensure consistent performance.

186005465 - XBridge Protein BEH C4 Method Validation Kit, 300Å, 3.5 µm, 4.6 mm X 100 mm, 3/pkg
Part Number: 186005465
Brand: XBridge

Products > Chromatography Consumables & Columns > Columns > HPLC Columns > XBridge Columns
% Carbon Load 8
Application Protein
Chemistry C4
Endcapped No
Endfitting Type Waters
Format Method Validation Kit
ID 4.6 mm
Length (mm) 100 mm
Mode Reversed-Phase
Particle Shape Spherical
Particle Size (dp) 3.5 µm
Particle Substrate Hybrid
Pore Size 300Å
QC Tested Protein
Silanol Activity Low
Surface Area 90
System HPLC
Technology BEH
USP Classification L26
Units in Package 3/pkg
pH Range 1 - 10

info

186006516 - ACQUITY UPLC Peptide BEH C18 Method Validation Kit, 130Å, 1.7 µm, 2.1 mm X 150 mm, 3/pkg

Part Number: 186006516

The ACQUITY BEH130 C18 Peptide Separation Technology (PST) columns have been specially optimized and QC tested for peptide separations. The small-pore (130Å) trifunctionally bonded BEH particle offers the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis.

186006516 - ACQUITY UPLC Peptide BEH C18 Method Validation Kit, 130Å, 1.7 µm, 2.1 mm X 150 mm, 3/pkg
Part Number: 186006516
Brand: ACQUITY UPLC

Products > Chromatography Consumables & Columns > Columns > UPLC Columns > ACQUITY UPLC Columns
% Carbon Load 18
Application Peptide
Chemistry C18
Endcapped Yes
Endfitting Type Waters
Format Method Validation Kit
ID 2.1 mm
Length (mm) 150 mm
Mode Reversed-Phase
Particle Shape Spherical
Particle Size (dp) 1.7 µm
Particle Substrate Hybrid
Pore Size 130Å
QC Tested Peptide
Silanol Activity Low
Surface Area 185
System UPLC/UHPLC
Technology BEH
USP Classification L1
Units in Package 3/pkg
pH Range 1 - 12

info

176003068 - ACQUITY UPLC Peptide CSH C18 Column Kit, Method Validation Kit, 130Å, 1.7 µm, 2.1 mm X 150 mm, 3/pkg

Part Number: 176003068

Waters ACQUITY UPLC CSH130 C18 1.7 µm particles contain a low and carefully-defined concentration of positive charges that yield comparatively excellent peak shape for peptides using either FA- or TFA-containing mobile phases. The fact that the performance of a CSH130 C18 column exhibits little dependence on strong ion pairing agents makes it ideal for LC or LC-MS applications. This kit consists of three ACQUITY UPLC CSH130 C18 columns packed with three different batches of sorbent and and one vial of Cytochrome C Digestion Standard (P/N 186006371). The standard is included to be able to immediately benchmark the column upon receipt and can be reordered as a separate part for continued monitoring. Each batch of digestion standard is prepared under strict quality control criteria confirmed on the included certificate of analysis.

176003068 - ACQUITY UPLC Peptide CSH C18 Column Kit, Method Validation Kit, 130Å, 1.7 µm, 2.1 mm X 150 mm, 3/pkg
Part Number: 176003068
Brand: ACQUITY UPLC

Products > Chromatography Consumables & Columns > Columns > UPLC Columns > ACQUITY UPLC Columns
% Carbon Load 15
Application Peptide
Chemistry C18
Endcapped Yes
Endfitting Type Waters
Format Column Kit
Method Validation Kit
ID 2.1 mm
Length (mm) 150 mm
Mode Reversed-Phase
Particle Shape Spherical
Particle Size (dp) 1.7 µm
Particle Substrate Hybrid
Pore Size 130Å
QC Tested Peptide
Silanol Activity Low
Surface Area 185
System UPLC/UHPLC
Technology CSH
USP Classification L1
Units in Package 3/pkg
pH Range 1 - 11

info

186004896 - ACQUITY UPLC Peptide BEH C18 Method Validation Kit, 130Å, 1.7 µm, 2.1 mm X 100 mm, 3/pkg

Part Number: 186004896

The ACQUITY BEH130 C18 Peptide Separation Technology (PST) columns have been specially optimized and QC tested for peptide separations. The small-pore (130Å) trifunctionally bonded BEH particle offers the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis.

186004896 - ACQUITY UPLC Peptide BEH C18 Method Validation Kit, 130Å, 1.7 µm, 2.1 mm X 100 mm, 3/pkg
Part Number: 186004896
Brand: ACQUITY UPLC

Products > Chromatography Consumables & Columns > Columns > UPLC Columns > ACQUITY UPLC Columns
% Carbon Load 18
Application Peptide
Chemistry C18
Endcapped Yes
Endfitting Type Waters
Format Method Validation Kit
ID 2.1 mm
Length (mm) 100 mm
Mode Reversed-Phase
Particle Shape Spherical
Particle Size (dp) 1.7 µm
Particle Substrate Hybrid
Pore Size 130Å
QC Tested Peptide
Silanol Activity Low
Surface Area 185
System UPLC/UHPLC
Technology BEH
USP Classification L1
Units in Package 3/pkg
pH Range 1 - 12

info

186004897 - ACQUITY UPLC Peptide BEH C18 Method Validation Kit, 300Å, 1.7 µm, 2.1 mm X 100 mm, 3/pkg

Part Number: 186004897

This kit consists of three ACQUITY UPLC BEH300 C18 PST columns packed with three different batches of sorbent.

186004897 - ACQUITY UPLC Peptide BEH C18 Method Validation Kit, 300Å, 1.7 µm, 2.1 mm X 100 mm, 3/pkg
Part Number: 186004897
Brand: ACQUITY UPLC

Products > Chromatography Consumables & Columns > Columns > UPLC Columns > ACQUITY UPLC Columns
% Carbon Load 18
Application Peptide
Chemistry C18
Endcapped Yes
Endfitting Type Waters
Format Method Validation Kit
ID 2.1 mm
Length (mm) 100 mm
Mode Reversed-Phase
Particle Shape Spherical
Particle Size (dp) 1.7 µm
Particle Substrate Hybrid
Pore Size 300Å
QC Tested Peptide
Silanol Activity Low
Surface Area 185
System UPLC/UHPLC
Technology BEH
USP Classification L1
Units in Package 3/pkg
pH Range 1 - 12

info

186006517 - ACQUITY UPLC Peptide BEH C18 Method Validation Kit, 300Å, 1.7 µm, 2.1 mm X 150 mm, 3/pkg

Part Number: 186006517

This kit consists of three ACQUITY UPLC BEH300 C18 PST columns packed with three different batches of sorbent.

186006517 - ACQUITY UPLC Peptide BEH C18 Method Validation Kit, 300Å, 1.7 µm, 2.1 mm X 150 mm, 3/pkg
Part Number: 186006517
Brand: ACQUITY UPLC

Products > Chromatography Consumables & Columns > Columns > UPLC Columns > ACQUITY UPLC Columns
% Carbon Load 18
Application Peptide
Chemistry C18
Endcapped Yes
Endfitting Type Waters
Format Method Validation Kit
ID 2.1 mm
Length (mm) 150 mm
Mode Reversed-Phase
Particle Shape Spherical
Particle Size (dp) 1.7 µm
Particle Substrate Hybrid
Pore Size 300Å
QC Tested Peptide
Silanol Activity Low
Surface Area 185
System UPLC/UHPLC
Technology BEH
USP Classification L1
Units in Package 3/pkg
pH Range 1 - 12

info

186004906 - XBridge Oligonucleotide BEH C18 Method Validation Kit, 130Å, 2.5 µm, 4.6 mm X 50 mm, 3/pkg

Part Number: 186004906

The reproducibility of a chromatographic column has a critical impact on the long-term reliability and robustness of an analytical method, and its reproducibility lies completely outside the user's control. With exceptional batch-to-batch and column-to-column reproducibility, Waters well-established particle and column manufacturing process control provides confidence in the long-term reliability of your analytical method. ACQUITY OST C18, 135A, 2.5um Method Validation Kit includes three batches of chromatographic media [derived from different base particles] to judge the quality, reliability, and consistency of your chromatographic method. Each batch of selected ACQUITY OST C18, 135A, 2.5um chemistry is specifically QC tested with a mixture of synthetic oligonucleotides to help ensure consistent performance.

186004906 - XBridge Oligonucleotide BEH C18 Method Validation Kit, 130Å, 2.5 µm, 4.6 mm X 50 mm, 3/pkg
Part Number: 186004906
Brand: XBridge

Products > Chromatography Consumables & Columns > Columns > Bioseparations Columns & Consumables > Oligonucleotides Separations > Oligonucleotides Separation Technology Columns
% Carbon Load 12
Application Oligonucleotide
Chemistry C18
Endcapped Yes
Endfitting Type Waters
Format Method Validation Kit
ID 4.6 mm
Length (mm) 50 mm
Mode Reversed-Phase
Particle Shape Spherical
Particle Size (dp) 2.5 µm
Particle Substrate Hybrid
Pore Size 130Å
QC Tested Oligonucleotide
Silanol Activity Low
Surface Area 185
System HPLC
Technology BEH
USP Classification L1
Units in Package 3/pkg
pH Range 1 - 12

info

186000195 - Symmetry C18 Method Validation Kit, 300Å, 3.5 µm, 4.6 mm X 150 mm, 3/pkg

Part Number: 186000195

Symmetry columns continues to set the standard for HPLC drug assays, giving you confidence in the long-term compliance of your HPLC methods. No need to compromise on peak shape, selectivity, column-to-column and batch-to-batch reproducibility, or other critical performance characteristics. Available in a variety of particle sizes, chemistries and dimensions.

186000195 - Symmetry C18 Method Validation Kit, 300Å, 3.5 µm, 4.6 mm X 150 mm, 3/pkg
Part Number: 186000195
Brand: Symmetry

Products > Chromatography Consumables & Columns > Columns > Bioseparations Columns & Consumables > Protein Separations > Symmetry300 Columns
% Carbon Load 8.5
Application Peptide
Chemistry C18
Endcapped Yes
Endfitting Type Waters
Format Method Validation Kit
ID 4.6 mm
Length (mm) 150 mm
Mode Reversed-Phase
Particle Shape Spherical
Particle Size (dp) 3.5 µm
Particle Substrate Silica
Pore Size 300Å
Silanol Activity Low
Surface Area 110
System HPLC
USP Classification L1
Units in Package 3/pkg
pH Range 2 - 8

info

WAT106181 - Symmetry C18 Cartridge Method Validation Kit, 300Å, 5 µm, 3.9 mm X 150 mm, 3/pkg

Part Number: WAT106181

Symmetry columns continues to set the standard for HPLC drug assays, giving you confidence in the long-term compliance of your HPLC methods. No need to compromise on peak shape, selectivity, column-to-column and batch-to-batch reproducibility, or other critical performance characteristics. Available in a variety of particle sizes, chemistries and dimensions.

WAT106181 - Symmetry C18 Cartridge Method Validation Kit, 300Å, 5 µm, 3.9 mm X 150 mm, 3/pkg
Part Number: WAT106181
Brand: Symmetry

Products > Chromatography Consumables & Columns > Columns > Bioseparations Columns & Consumables > Protein Separations > Symmetry300 Columns
% Carbon Load 8.5
Application Peptide
Chemistry C18
Endcapped Yes
Format Cartridge Method Validation Kit
ID 3.9 mm
Length (mm) 150 mm
Mode Reversed-Phase
Particle Shape Spherical
Particle Size (dp) 5 µm
Particle Substrate Silica
Pore Size 300Å
Silanol Activity Low
Surface Area 110
System HPLC
USP Classification L1
Units in Package 3/pkg
pH Range 2 - 8

info

WAT054454 - Symmetry C18 Cartridge Method Validation Kit, 100Å, 5 µm, 4.6 mm X 150 mm, 3/pkg

Part Number: WAT054454

This kit consists of three Symmetry C18 columns packed with three different batches of sorbent. This product needs to be used in conjunction with other parts. Please see the section below on related parts for more information.

WAT054454 - Symmetry C18 Cartridge Method Validation Kit, 100Å, 5 µm, 4.6 mm X 150 mm, 3/pkg
Part Number: WAT054454
Brand: Symmetry

Products > Chromatography Consumables & Columns > Columns > HPLC Columns
% Carbon Load 19.1
Chemistry C18
Endcapped Yes
Format Cartridge Method Validation Kit
ID 4.6 mm
Length (mm) 150 mm
Mode Reversed-Phase
Particle Shape Spherical
Particle Size (dp) 5 µm
Particle Substrate Silica
Pore Size 100Å
Silanol Activity Low
Surface Area 335
System HPLC
USP Classification L1
Units in Package 3/pkg
pH Range 2 - 8

info

186002314 - Atlantis Silica HILIC Method Validation Kit, 100Å, 5 µm, 4.6 mm X 150 mm, 3/pkg

Part Number: 186002314

Atlantis HILIC Silica columns are used in combination with high organic mobile phases (>80% acetonitrile) to provide retention of analytes that are simply too polar to be retained by reverse-phase chromatography. HILIC separation mechanisms provide orthogonal analyte selectivity compared to traditional chromatographic reversed-phase approaches.

186002314 - Atlantis Silica HILIC Method Validation Kit, 100Å, 5 µm, 4.6 mm X 150 mm, 3/pkg
Part Number: 186002314
Brand: Atlantis

Products > Chromatography Consumables & Columns > Columns > HPLC Columns
Chemistry HILIC
Endcapped No
Endfitting Type Waters
Format Method Validation Kit
ID 4.6 mm
Length (mm) 150 mm
Mode Reversed-Phase
Particle Shape Spherical
Particle Size (dp) 5 µm
Particle Substrate Silica
Pore Size 100Å
Silanol Activity High
Surface Area 330
System HPLC
Technology Silica
USP Classification L3
Units in Package 3/pkg
pH Range 1 - 5

info

186002315 - Atlantis Silica HILIC Method Validation Kit, 100Å, 3 µm, 4.6 mm X 150 mm, 3/pkg

Part Number: 186002315

Atlantis HILIC Silica columns are used in combination with high organic mobile phases (>80% acetonitrile) to provide retention of analytes that are simply too polar to be retained by reverse-phase chromatography. HILIC separation mechanisms provide orthogonal analyte selectivity compared to traditional chromatographic reversed-phase approaches.

186002315 - Atlantis Silica HILIC Method Validation Kit, 100Å, 3 µm, 4.6 mm X 150 mm, 3/pkg
Part Number: 186002315
Brand: Atlantis

Products > Chromatography Consumables & Columns > Columns > HPLC Columns
Chemistry HILIC
Endcapped No
Endfitting Type Waters
Format Method Validation Kit
ID 4.6 mm
Length (mm) 150 mm
Mode Reversed-Phase
Particle Shape Spherical
Particle Size (dp) 3 µm
Particle Substrate Silica
Pore Size 100Å
Silanol Activity High
Surface Area 330
System HPLC
Technology Silica
USP Classification L3
Units in Package 3/pkg
pH Range 1 - 5

info

186006026 - ACQUITY UPLC HSS C18 Method Validation Kit, 100Å, 1.8 µm, 2.1 mm X 150 mm, 3/pkg

Part Number: 186006026

This kit consists of three ACQUITY HSS C18 columns packed with three different batches of sorbent.

186006026 - ACQUITY UPLC HSS C18 Method Validation Kit, 100Å, 1.8 µm, 2.1 mm X 150 mm, 3/pkg
Part Number: 186006026
Brand: ACQUITY UPLC

Products > Chromatography Consumables & Columns > Columns > UPLC Columns > ACQUITY UPLC Columns
% Carbon Load 11
Chemistry C18
Endcapped Yes
Endfitting Type Waters
Format Method Validation Kit
ID 2.1 mm
Length (mm) 150 mm
Mode Reversed-Phase
Particle Shape Spherical
Particle Size (dp) 1.8 µm
Particle Substrate Silica
Pore Size 100Å
Silanol Activity Low
Surface Area 230
System UPLC/UHPLC
Technology HSS
USP Classification L1
Units in Package 3/pkg
pH Range 2 - 8

info

186002676 - SunFire C18 Method Validation Kit, 100Å, 3.5 µm, 4.6 mm X 150 mm, 3/pkg

Part Number: 186002676

This kit consists of three Sunfire C18 columns packed with three different batches of sorbent.

186002676 - SunFire C18 Method Validation Kit, 100Å, 3.5 µm, 4.6 mm X 150 mm, 3/pkg
Part Number: 186002676
Brand: SunFire

Products > Chromatography Consumables & Columns > Columns > HPLC Columns
% Carbon Load 16
Chemistry C18
Endcapped Yes
Endfitting Type Waters
Format Method Validation Kit
ID 4.6 mm
Length (mm) 150 mm
Mode Reversed-Phase
Particle Shape Spherical
Particle Size (dp) 3.5 µm
Particle Substrate Silica
Pore Size 100Å
Silanol Activity Low
Surface Area 340
System HPLC
USP Classification L1
Units in Package 3/pkg
pH Range 2 - 8

info

186002675 - SunFire C18 Method Validation Kit, 100Å, 3.5 µm, 4.6 mm X 100 mm, 3/pkg

Part Number: 186002675

This kit consists of three Sunfire C18 columns packed with three different batches of sorbent.

186002675 - SunFire C18 Method Validation Kit, 100Å, 3.5 µm, 4.6 mm X 100 mm, 3/pkg
Part Number: 186002675
Brand: SunFire

Products > Chromatography Consumables & Columns > Columns > HPLC Columns
% Carbon Load 16
Chemistry C18
Endcapped Yes
Endfitting Type Waters
Format Method Validation Kit
ID 4.6 mm
Length (mm) 100 mm
Mode Reversed-Phase
Particle Shape Spherical
Particle Size (dp) 3.5 µm
Particle Substrate Silica
Pore Size 100Å
Silanol Activity Low
Surface Area 340
System HPLC
USP Classification L1
Units in Package 3/pkg
pH Range 2 - 8

info

186007156 - CORTECS UPLC C18 Method Validation Kit, 90Å, 1.6 µm, 2.1 mm X 30 mm, 3/pkg

Part Number: 186007156

CORTECS C18 Columns are general purpose, high-efficiency columns based on a solid-core particle that offer balanced retention of acids, bases and neutrals at low and mid-range pH.

186007156 - CORTECS UPLC C18 Method Validation Kit, 90Å, 1.6 µm, 2.1 mm X 30 mm, 3/pkg
Part Number: 186007156
Brand: CORTECS

Products > Chromatography Consumables & Columns
% Carbon Load 6.6
Chemistry C18
Endcapped Yes
Endfitting Type Parker-style
Format Method Validation Kit
ID 2.1 mm
Length (mm) 30 mm
Mode Reversed-Phase
Particle Shape Spherical
Particle Size (dp) 1.6 µm
Particle Substrate Solid-Core
Pore Size 90Å
Silanol Activity Low
Surface Area 100
System UPLC/UHPLC
Technology UPLC
USP Classification L1
Units in Package 3/pkg
pH Range 2 - 8

info

186007159 - CORTECS UPLC C18 Method Validation Kit, 90Å, 1.6 µm, 2.1 mm X 100 mm, 3/pkg

Part Number: 186007159

CORTECS C18 Columns are general purpose, high-efficiency columns based on a solid-core particle that offer balanced retention of acids, bases and neutrals at low and mid-range pH.

186007159 - CORTECS UPLC C18 Method Validation Kit, 90Å, 1.6 µm, 2.1 mm X 100 mm, 3/pkg
Part Number: 186007159
Brand: CORTECS

Products > Chromatography Consumables & Columns
% Carbon Load 6.6
Chemistry C18
Endcapped Yes
Endfitting Type Parker-style
Format Method Validation Kit
ID 2.1 mm
Length (mm) 100 mm
Mode Reversed-Phase
Particle Shape Spherical
Particle Size (dp) 1.6 µm
Particle Substrate Solid-Core
Pore Size 90Å
Silanol Activity Low
Surface Area 100
System UPLC/UHPLC
Technology UPLC
USP Classification L1
Units in Package 3/pkg
pH Range 2 - 8

info

186007157 - CORTECS UPLC C18 Method Validation Kit, 90Å, 1.6 µm, 2.1 mm X 50 mm, 3/pkg

Part Number: 186007157

CORTECS C18 Columns are general purpose, high-efficiency columns based on a solid-core particle that offer balanced retention of acids, bases and neutrals at low and mid-range pH.

186007157 - CORTECS UPLC C18 Method Validation Kit, 90Å, 1.6 µm, 2.1 mm X 50 mm, 3/pkg
Part Number: 186007157
Brand: CORTECS

Products > Chromatography Consumables & Columns
% Carbon Load 6.6
Chemistry C18
Endcapped Yes
Endfitting Type Parker-style
Format Method Validation Kit
ID 2.1 mm
Length (mm) 50 mm
Mode Reversed-Phase
Particle Shape Spherical
Particle Size (dp) 1.6 µm
Particle Substrate Solid-Core
Pore Size 90Å
Silanol Activity Low
Surface Area 100
System UPLC/UHPLC
Technology UPLC
USP Classification L1
Units in Package 3/pkg
pH Range 2 - 8

info

186007158 - CORTECS UPLC C18 Method Validation Kit, 90Å, 1.6 µm, 2.1 mm X 75 mm, 3/pkg

Part Number: 186007158

CORTECS C18 Columns are general purpose, high-efficiency columns based on a solid-core particle that offer balanced retention of acids, bases and neutrals at low and mid-range pH.

186007158 - CORTECS UPLC C18 Method Validation Kit, 90Å, 1.6 µm, 2.1 mm X 75 mm, 3/pkg
Part Number: 186007158
Brand: CORTECS

Products > Chromatography Consumables & Columns
% Carbon Load 6.6
Chemistry C18
Endcapped Yes
Endfitting Type Parker-style
Format Method Validation Kit
ID 2.1 mm
Length (mm) 75 mm
Mode Reversed-Phase
Particle Shape Spherical
Particle Size (dp) 1.6 µm
Particle Substrate Solid-Core
Pore Size 90Å
Silanol Activity Low
Surface Area 100
System UPLC/UHPLC
Technology UPLC
USP Classification L1
Units in Package 3/pkg
pH Range 2 - 8